Multiplex Immunoassays using Bio-plex assays

Multiplex immunoassays allow simultaneous detection and quantification of multiple analytes in a single sample from a variety of biological specimens including but not limited to serum, plasma, CSF, cell culture supernatants, sputum.

Luminex xMap technology is a bead-based multiplexed immunoassay system in a microplate format. The system can simultaneously detect many targets in a single sample — up to 500, depending on system design. Using xMAP technology, the microspheres used in the Luminex system have different spectral addresses (color codes). These unique spectral addresses are created by internally labeling beads with different ratios of two fluorophores, one in a red wavelength and the other in the far red.

Bio-Plex assays use antibodies coupled to the magnetic beads. In the kits, every antibody to a different target is covalently attached to beads with a different single spectral address, giving each target its own unique address.

In an assay using the Luminex system, the beads are incubated with a sample. After washing, the beads are incubated with a mixture of biotinylated antibodies against all the targets. After another wash, the beads are incubated with the reporter streptavidin-PE. For targets that are present in the sample, a sandwich is generated that is composed of: bead with attached target-specific antibody + target + biotinylated target- specific antibody + streptavidin reporter. Using a dual laser system, the signature of each bead is identified, and the presence and intensity of reporter associated with the bead is detected simultaneously. This gives information about both the identity and concentration of targets in the sample.

Bio-Plex Pro™ Human Cytokine 27-plex Assay

The Bio-Plex Pro Human Cytokine 27-Plex Immunoassay is a 1 x 96-well kit that includes coupled magnetic beads, detection antibodies, standards, assay buffer, wash buffer, sample diluent, standard diluent, detection antibody diluent, streptavidin-PE, a flat bottom plate, and sealing tape. This multiplex assay detects:

FGF basic, Eotaxin, G-CSF, GM-CSF, IFN-γ, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IP-10, MCP-1 (MCAF), MIP-1α, MIP-1β, PDGF-BB, RANTES, TNF-α, VEGF.

Based on 6.5 µm magnetic beads, it is compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Bio-Plex Pro Human Cytokine Screening Panel, 48-Plex

The Bio-Plex Pro™ Human Cytokine Screening Panel enables you to interrogate 48 cytokine and chemokine cell signaling molecules in complex extracellular events and signaling pathways involved in heart disease, Alzheimer’s disease, autoimmunity and allergy, infectious diseases, inflammation, cancer, and neurodegeneration. This high-performance, broad screening panel consists of a biologically-relevant collection of adaptive immunity cytokines, pro-inflammatory cytokines, and anti-inflammatory cytokines all in a single well. Achieve accurate, actionable results with the Bio-Plex Pro™ Human Cytokine Screening Panel, 48-plex. This panel includes:

FGF basic, Eotaxin, G-CSF, GM-CSF, IFN-γ, IL-1β, IL-1ra, IL-1α, IL-2Rα, IL-3, IL-12 (p40), IL-16, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, GRO-α, HGF, IFN-α2, LIF, MCP-3, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IP-10, MCP-1 (MCAF), MIG, β-NGF, SCF, SCGF-β, SDF-1α, MIP-1α, MIP-1β, PDGF-BB, RANTES, TNF-α, VEGF, CTACK, MIF, TRAIL, IL-18, M-CSF, TNF-β.

Bio-Plex Pro™ Human Cytokine 17-plex Assay: G-CSF, GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, MCP-1 (MCAF), MIP-1β, TNF-α.

Bio-Plex Pro™ Human Cytokine 8-plex Assay: GM-CSF, IFN-γ, IL-2, IL-4, IL-6, IL-8, IL-10, TNF-α.

Bio-Plex Pro™ Human Cytokine Th1/Th2 Assay: GM-CSF, IFN-γ, IL-2, IL-4, IL-5, IL-10, IL-12 (p70), IL-13, TNF-α.

Bio-Plex Pro™ TGF-β 3-plex Assay: TGF-β1, TGF-β2, TGF-β3. This assay is compatible in human, mouse, and rat models in diverse matrices like serum, plasma, urine, and milk.

Bio-Plex Pro Human Acute Phase Multiplex Assays: Disease research involving the study of inflammation, sepsis, cardiovascular disease, and diabetes often requires the measurement of multiple acute phase biomarkers. Bio-Plex Pro human acute phase immunoassays deliver accurate and reproducible measurements of nine of the most commonly measured biomarkers, using the Bio-Plex 200 system. The multiplex immunoassays have optimal performance in serum, plasma, and tissue culture supernatant. Bio-Plex offers a 4-plex panel and a 5-plex panel, as well as a complete kit which contains both panels. 

The 5-plex panel detects: Ferritin, Fibrinogen, Procalcitonin, Serum amyloid A, Tissue plasminogen activator. 

The 4-plex panel detects: α-2-macroglobin, C-reactive protein (CRP), Haptoglobin, Serum amyloid P.

Benefits of Bio-Plex Pro™ Assays:

  • Comprehensive panel coverage: Detect the most often researched and biologically relevant cytokines in a single well
  • Broad dynamic range: Quantitate both endogenous and diseased/treated conditions in the same assay
  • Best-in-class performance: Achieve a higher level of sensitivity and analyte detection with optimized reagents
  • Reduced turnaround time: Save time with a fast protocol
  • Reproducible results: Gain confidence in your experiments with a robust, magnetic bead-based workflow