HDX-MS Protein Structural Analysis

HDX-MS analysis: It can be used to obtain information on your protein structure, its interaction sites (e.g., protein-protein or protein-ligand), Epitope mapping, allosteric effects, intrinsic disorder, and conformational changes induced by post-translational modifications (PTMs). 

HDX-MS has the unique advantage of not being limited by the size of proteins or protein complexes. This technique is highly sensitive, and can able to detect co-existing protein conformations.

  • We provide services for Protein Structural Analysis using Hydrogen-Deuterium exchange (HDX) mass spectrometry (MS).
  • HDX-MS has become a popular and key technique for monitoring structural and dynamic aspects of proteins in solution.
  • This approach relies on the fact that exposure of a protein to D(2)O induces rapid amide H → D exchange in disordered regions that lack stable hydrogen-bonding.