Post Translational Modification (PTM) Analysis

1. What modification ?
2. Protein or proteome ?
3. Identify site in sequence ?
4. Occupancy ?

  • Post-translational modifications (PTMs) are covalent and generally enzymatic modifications of proteins following protein biosynthesis. There are more than 400 types of PTMs affecting many aspects of the protein function.
  • For certain modifications (phosphorylation, glycosylation etc.) there are standardized methods available for enrichment and data processing. For other modifications method development might be required.  

Protein analysis:

Detailed analysis can be made on a majority of different PTMs. Distribution and occupancy of different proteforms can be generated. Site specificity etc. can be identified. 

Proteome analysis:

Limited to specific PTMs with developed and standardized  enrichment methods (phosphorylation, glycosylation etc). Specific PTM sites can be identified but information of protein occupancy is limited, but can somewhat be compared relatively between two different states etc.

PTMs Analysis Types/Strategies